Measuring In Vivo Protein Half-Life
نویسندگان
چکیده
منابع مشابه
Measuring in vivo protein half-life.
Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and ...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2011
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2011.03.014